Menthol based hydrophobic deep eutectic solvent for extraction and purification of ergosterol using response surface methodology

Vitamin D deficiency has been linked to various diseases, but could be rectified via fortified food stuffs or supplementation. In this study 39 different hydrophobic deep eutectic solvents were evaluated for green extraction of ergosterol from mushroom. Extraction parameters (e.g. time, solvent volume) optimized using response surface methodology (RSM) and a maximum yield 6995.00 µg ergosterol/g dry weight mushroom was attained with menthol: pyruvic acid. The extracted purified novel the reused six cycles, while retaining efficiency (up 28%). exposed ultra-violet radiation conversion ergocalciferol (vitamin D2) resulting in that equivalent 2142.01 µg/g